Investigation of inducible transposon to remove selection marker
Date Issued
2005
Date
2005
Author(s)
Li, Kuan-Te
DOI
zh-TW
Abstract
The maize transposable element Ac can move to new location within genome. In this work, an Ac–based inducible transposon was constructed to develop a one-time gene expression system. Firstly, the 5’ end of the Ac was inserted in the first intron of the modified rice EPSPS gene, which triggered by the CaMV35S promoter. The inducible transposon was completed by locating the PR-1a promoter-transposase fusion and the CaMV35S promoter was between the 5’ and 3’ ends. Thus, this inducible transposon contains not only the PR-TPase fusion but also the first exon of the modified EPSPS gene and its promoter. This construct, which termed as KCEH, was introduced into rice. The modified EPSPS gene can be transcribed normally and allow the transgenic rice to be Glyphosate-resistant. After the Glyphosate screening, the transgenic rice plants were treated with salicylic acid for the excision of the inducible transposon. As a result of this, the modified EPSPS gene was truncated by losing its first exon and the 35S promoter. Emphasis has been placed on the theoretical and practical aspects of the approach that may be relevant to its application to other gene expression regulatory system. It provides a means to investigate a one-time gene expression system.
Subjects
篩選標記基因
可誘導轉位子
篩選標記基因去除
嘉磷塞
水稻
selection marker
inducible transposon
marker-free
glyphosate
rice
5-enolpyruvylshikimate-3-phosphate synthase
Type
thesis
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