Zebrafish heparin-binding neurotrophic factor enhances neurite outgrowth during its development
Journal
Biochemical and Biophysical Research Communications
Journal Volume
321
Journal Issue
2
Pages
502-509
Date Issued
2004
Date
2004
Author(s)
Chang, Ming-Huang
Huang, Chang-Jen
Hwang, Sheng-Ping L.
Lu, I-Ching
Lin, Chien-Ming
Chou, Chih-Ming
Abstract
Heparin-binding neurotrophic factor (HBNF) is a secreted heparin-binding protein containing highly basic and cysteine-rich amino acid residues. In this study, we cloned the full-length HBNF cDNA from zebrafish and determined its genomic structure by bioinformatics analysis. Zebrafish HBNF gene is composed of five exons and four introns spanning approximately 82kb. RT-PCR analysis revealed that zebrafish HBNF transcript was highly expressed in adult brain and intestine tissues while less in other tissues. During embryogenesis, zebrafish HBNF transcript was observed to be moderately expressed at earlier stages with a gradual decline. Higher expression level was observed after hatching and maintaining this level into adulthood. The overall amino acid sequence of zebrafish HBNF shows 60% identity to human HBNF, but with approximately 40% identity to other midkine proteins. Like mammalian homolog, zebrafish HBNF could induce significant neurite outgrowth in PC12 cells without NGF stimulation. In addition, zebrafish HBNF was able to enhance extensive neurite outgrowth in zebrafish embryos during embryogenesis. In summary, a feasible in vivo assay for neurite outgrowth was established in zebrafish. ? 2004 Elsevier Inc.
Subjects
Heparin-binding neurotrophic factor; Neurite outgrowth; Neuron-specific promoter; PC12 cells; Zebrafish
SDGs
Other Subjects
complementary DNA; midkine; nerve growth factor; pleiotrophin; zebrafish protein; carrier protein; cytokine; messenger RNA; pleiotrophin; animal cell; animal tissue; article; bioinformatics; brain; cell strain; controlled study; developmental stage; embryo; embryo development; exon; feasibility study; gene structure; genetic transcription; genome analysis; hatching; human versus animal comparison; in vitro study; intestine; intron; mammal; molecular cloning; nerve fiber growth; nonhuman; nucleotide sequence; priority journal; protein localization; rat; reverse transcription polymerase chain reaction; sequence homology; tissue distribution; zebra fish; amino acid sequence; animal; cell size; chemistry; cytology; drug effect; gene expression profiling; gene expression regulation; genetics; human; metabolism; molecular genetics; neurite; prenatal development; sequence alignment; zebra fish; Danio rerio; Mammalia; Amino Acid Sequence; Animals; Carrier Proteins; Cell Size; Cloning, Molecular; Cytokines; DNA, Complementary; Embryo; Gene Expression Profiling; Gene Expression Regulation, Developmental; Humans; Molecular Sequence Data; Neurites; PC12 Cells; Rats; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger; Sequence Alignment; Zebrafish
Type
journal article
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