A RAPD Specific to Rice Wild Species with B Genome
Date Issued
2007
Date
2007
Author(s)
Fang, Shao-An
DOI
zh-TW
Abstract
Molecular markers are useful in discriminating specific constituents of genome and monitoring genomic introgression of interspecific hybrids. In this study, we use 12 rice materials, including nine Oryza species, both cultivated and wild species of rice, which belong to six genome types. We isolate and characterize two genome specific RAPDs, named Opun225 and Opun246, from Oryza punctata Kotschy ex Steud (W1593), an African native wild rice. The Opun225 sequence is 874 base pair in length and a low-copy sequence present in genome and is demonstrated as a repetitive sequence specific to a diploid Oryza species with B genome. On the other hand, the Opun246 is 2084 bp in length and estimated at 6320 copies in O. punctata (W1593) haploid genome. Sequence comparison analysis by BLAST found a few homologues of Opun246 in EMBL-GenBank database. Among those homologues, a relatively high similarity was found in the position ~1200 nucleotides regions at the 5’ ends, but a low similarity in the rest of the nucleotides at the 3’ends. In addition, the Opun246 sequence at position 75~1266 nucleotides with 75% identity to a putative retroelements. The results of Southern hybridization indicated that the repetitive Opun246 sequences dispersed throughout the entire genome of specues with B genome. The results of fluorescent in situ hybridization (FISH) indicated that the repetitive Opun246 sequence dispersed to the heterochromatin throughout chromosomes in the whole genome. Furthermore, obvious Opun246 sequences were detected near the centrimeric and terminal regions at chromosomes of O. punctata (W15777). The Opun246 sequence were detected near the terminal regions on part of chromosomes, which might be restricted to B genome originated chromosomes in O. punctata (W1564) and O. minuta (W0045) with BBCC.
Subjects
螢光原位雜交技術
野生稻Oryza punctata
重複序列
物種專一性逢機引子多型性標誌
Oryza punctata
repetitive sequence
species-specific RAPD
fluorescent in situ hybridization (FISH)
Type
thesis
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