Genotoxicity tests of poly(styrene-co-maleic anhydride)-coated silver nanoparticles in vivo and in vitro
Journal
Journal of Experimental Nanoscience
Journal Volume
10
Journal Issue
6
Pages
449-457
Date Issued
2015
Author(s)
Chen, Y.T.
Wu, J.H.
Tsai, F.J.
Chang, Y.W.
Hsu, S.H.
Lin, J.J.
Sue, H.L.
Liao, J.W.
Abstract
Nanosilver is thought to hold potential for use in medical materials. The safety of newly developed poly(styrene-co-maleic anhydride)-coated silver nanoparticles (SMA-AgNPs) requires investigation. In this study, three in vitro and in vivo experiments for investigating genetic toxicity–the Ames test, a micronucleus assay, and a chromosome aberration test–were conducted. Results from the Ames testing showed SMA-AgNPs to have a negative effect, either with or without S9 metabolism. In addition, SMA-AgNPs increased the number of reticulocytes and micronuclei in reticulocytes at 48 and 72 h after treatment. Indeed, SMA-AgNPs induced significant changes in the chromosomal aberration rate in CHO-K1 (Chinese hamster ovary cell clone K1) cells. In conclusion, SMA-AgNPs did cause DNA damage in terms of chromosomal aberration and may have a potential genotoxic effect in certain applications.
Type
journal article