Fluorescence lifetime imaging microscopy of nanodiamonds in vivo
Journal
Proceedings of SPIE - The International Society for Optical Engineering
Journal Volume
8635
Date Issued
2013
Author(s)
Abstract
The negatively charged nitrogen-vacancy (NV–) center in bulk diamond is a photostable fluorophore with a radiative lifetime of 11.6 ns at room temperature. The lifetime substantially increases to ~20 ns for diamond nanoparticles (size ~ 100 nm) suspended in water due to the change in refractive index of the surrounding medium of the NV– centers. This fluorescence decay time is much longer than that (typically 1 − 4 ns) of endogenous and exogenous fluorophores commonly used in biological imaging, making it possible to detect NV–-containing nanodiamonds in vivo at the single particle level by fluorescence lifetime imaging microscopy (FLIM). We demonstrate the feasibility of this approach using Caenorhabditis elegans (C. elegans) as a model organism.
Event(s)
Advances in Photonics of Quantum Computing, Memory, and Communication VI
SDGs
Type
conference paper