Spectrum resolved fluorescence imaging in multi-focal volume holographic microscopy
Journal
Progress in Biomedical Optics and Imaging - Proceedings of SPIE
Journal Volume
7891
Date Issued
2011
Author(s)
Abstract
A real-time three dimensional (3D) fluorescence imaging system incorporating wavelength-coded and multiplexed holographic gratings is presented. Holographic gratings formed in thick Phenanthrenquinone- (PQ-) Doped Poly (methyl methacrylate) (PMMA) have narrowband spectral-spatial transmittance filtering properties to generate wavelengthspectrum selective multi-focal planes within a biological object. We demonstrate the imaging modality to obtain laserinduced fluorescent tissue structures from different depths at the excitation wavelength of 355nm.
Type
conference paper