Glycan Array on Aluminum Oxide-Coated Glass Slides through Phosphonate Chemistry
Resource
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 132(38), 13371-13380
Journal
Journal of the American Chemical Society
Pages
13371-13380
Date Issued
2010
Date
2010
Author(s)
Chang, Shih-Huang
Han, Jeng-Liang
Tseng, Susan Y.
Lin, Chin-Wei
Lin, Yu-Chen
Jeng, Wen-Yih
Wang, Andrew H.-J.
Wu, Chung-Yi
Wong, Chi-Huey
Abstract
A new type of glycan array covalently or noncovalently attached to aluminum oxide-coated glass (ACG) slides has been developed for studies of enzymatic reactions and protein binding. To prepare the noncovalent array, glycans with a polyfluorinated hydrocarbon (-C(8)F(17)) tail are spotted robotically onto the ACG slide surface containing a layer of polyfluorinated hydrocarbon terminated with phosphonate. After incubation and washing, the noncovalent array can be characterized by MS-TOF via ionization/desorption at a low laser energy without addition of matrix. A representative cellotetraose array was developed to study the activity and specificity of different cellulases and to differentiate the exo- and endoglucanase activities. To prepare the covalent array, glycans with a phosphonic acid tail were synthesized and spotted robotically onto the ACG slide surface. After incubation, the slides can be used directly for quantitative protein binding analysis. Compared to the preparation of glycan arrays on glass slides and other surfaces, this method of arraying using phosphonic acid reacting with ACG is more direct, convenient, and effective and represents a new platform for the high-throughput analysis of protein-glycan interactions.
Type
journal article
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