Nanomaterial-based surface-assisted laser desorption/ionization mass spectrometry of peptides and proteins
Journal
Journal of the American Society for Mass Spectrometry
Journal Volume
21
Journal Issue
7
Pages
1204
Date Issued
2010-07
Author(s)
Abstract
We have investigated six nanomaterials for their applicability as surfaces for the analyses of peptides and proteins using surface-assisted laser desorption/ionization mass spectrometry (SALDI-MS). Gold nanoparticles (NPs) were useful nanomaterials for small analytes (e.g., glutathione); Pt nanosponges and Fe(3)O(4) NPs were efficient nanomaterials for proteins, with an upper detectable mass limit of ca. 25 kDa. Nanomaterials have several advantages over organic matrices, including lower limits of detection for small analytes and lower batch-to-batch variations (fewer problems associated with "sweet spots"), when used in laser desorption/ionization mass spectrometry.
Subjects
NANOPARTICLE MATRICES; AFFINITY PROBES; NANODOTS; CARBON; AU
Publisher
SPRINGER
Type
journal article