Plasma Clusterin Increased Prior to Small for Gestational Age (SGA) Associated With Preeclampsia and Decreased Prior to SGA in Normotensive Pregnancies
Journal
Reproductive Sciences
Journal Volume
19
Journal Issue
6
Date Issued
2012
Author(s)
Abstract
In our search for early biomarkers for the pregnancy complicationssmall for gestational age (SGA) and preeclampsia (PE) we analysed plasma from 19-21 weeks gestation in women recruited into the SCOPE study, a prospective cohort of nulliparous women, by differential in gel electrophoresis (DIGE). DIGE revealed the differential expression of clusterin levels and its isoforms in top6-depleted plasma of women who delivered an SGA infant but remained normotensive (SGA-NT; N = 8) compared to healthy women with an uncomplicated pregnancy outcome (Controls, N = 8). Immunosorbent enzyme-linked assay (ELISA) showed that compared to plasma clusterin levels from healthy controls [71.1 (SD 12.4) μg/mL, n = 39], clusterin was decreased in SGA-NT [58.3 (SD 11.7), N = 20, P < 0.0001], increased in women with SGA and PE [81.5 (SD 14.8), N = 20, P < 0.01], but similar in PE alone [71.2 (SD 9.4)g/ml, P = 1.0]. Screening for clusterin levels and/or its different isoformsmay be useful in mid-pregnancy to identify women who subsequently develop SGA but remain normotensive or who develop preeclampsia with SGA. © The Author(s) 2012.
Subjects
clusterin | difference in gel electrophoresis | DIGE | plasma | preeclampsia | small for gestational age
SDGs
Publisher
Springer Science and Business Media
Type
journal article