Distinct Rac Activation Pathways Control Caenorhabditis elegans Cell Migration and Axon Outgrowth
Resource
Developmental Biology 250 (1): 145-155
Journal
Developmental Biology
Journal Volume
250
Journal Issue
1
Pages
145-155
Date Issued
2002
Date
2002
Author(s)
Cheng, Ting-Wen
Lee, Ming-Chia
Weng, Nei-Ying
Wu, Chia-Hsuan
Chen, Yi-Cheng
Chen, Chin-Pei
Wu YC
Abstract
Rac GTPases act as molecular switch in various morphogenic events. However, the regulation of their activities during the development of multicellular organisms is not well understood. Caenorhabditis elegans rac genes ced-10 and mig-2 have been shown to act redundantly to control P cell migration and the axon outgrowth of D type motoneurons. We showed that ced-10 and mig-2 also control amphid axon outgrowth and amphid dendrite fasciculation in a redundant fashion. Our biochemical and genetic data indicate that unc-73, which encodes a protein related to Trio-like guanine nucleotide exchange factor, acts as a direct activator of ced-10 and mig-2 during P cell migration and axon outgrowth of D type motoneurons and amphid sensory neurons. Furthermore, rac regulators ced-2/crkII and ced-5/dock180 function genetically upstream of ced-10 and mig-2 during axon outgrowth of D type motoneurons and act upstream of mig-2 but not ced-10 during P cell migration. However, neither ced-2/crkII nor ced-5/dock180 is involved in amphid axon outgrowth. Therefore, distinct rac regulators control ced-10 and mig-2 differentially in various cellular processes. © 2002 Elsevier Science (USA).
Subjects
Axon outgrowth; ced-10; ced-12; ced-2; ced-5; Cell migration; Guanine nucleotide exchange factor; mig-2; Rac; unc-73
Other Subjects
guanine nucleotide exchange factor; Rac protein; article; biochemistry; Caenorhabditis elegans; ced 10 gene; ced 5 gene; cell migration; controlled study; crkII gene; dock180 gene; gene; genetics; mig 2 gene; motoneuron; nerve fiber; nonhuman; priority journal; rac gene; unc 73 gene; Caenorhabditis; Caenorhabditis elegans
Type
journal article
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