Determination of the cell tropism of serotype 1 feline infectious peritonitis virus using the spike affinity histochemistry in paraffin-embedded tissues
Journal
Microbiology and Immunology
Journal Volume
61
Journal Issue
8
Pages
318-327
Date Issued
2017
Author(s)
Abstract
Unlike for serotype II feline coronaviruses (FCoV II), the cellular receptor for serotype I FCoV (FCoV I), the most prevalent FCoV serotype, is unknown. To provide a platform for assessing the pattern by which FCoV I attaches to its host receptor(s), HEK293 cell lines that stably express the ectodomains of the spike (S) proteins derived from a FCoV I feline enteric coronavirus strain UU7 (FECV UU7) and a feline infectious peritonitis virus strain UU4 (FIPV UU4) were established. Using the recombinant S proteins as probes to perform S protein affinity histochemistry in paraffin-embedded tissues, although no tissue or enteric binding of FECV UU7 S protein was detected, it was found that by immunohistochemistry that the tissue distribution of FIPV UU4 S protein-bound cells correlated with that of FIPV antigen-positive cells and lesions associated with FIP and that the affinity binding of FIPV UU4 S protein on macrophages was not affected by enzymatic removal of host cell-surface sialic acid with neuraminidase. These findings suggest that a factor(s) other than sialic acid contribute(s) to the macrophage tropism of FIPV strain UU4. This approach allowed obtaining more information about both virus–host cell interactions and the biological characteristics of the unidentified cellular receptor for FCoV I. ? 2017 The Societies and John Wiley & Sons Australia, Ltd
Subjects
sialic acid; vitronectin; coronavirus spike glycoprotein; n acetylneuraminic acid; protein binding; virus receptor; animal tissue; Article; binding affinity; cell surface; controlled study; Feline infectious peritonitis virus; histochemistry; human; human cell; macrophage; male; nonhuman; pathogenesis; protein binding; protein domain; protein expression; viral tropism; virus infection; virus strain; animal; cat; cell line; chemistry; Feline coronavirus; genetics; HEK293 cell line; host pathogen interaction; metabolism; physiology; serotype; viral tropism; virology; virus attachment; Animals; Cats; Cell Line; Coronavirus, Feline; HEK293 Cells; Host-Pathogen Interactions; Humans; Macrophages; N-Acetylneuraminic Acid; Protein Binding; Receptors, Virus; Serogroup; Spike Glycoprotein, Coronavirus; Viral Tropism; Virus Attachment
cell tropism; feline infectious peritonitis virus; histochemistry; spike
SDGs
Type
journal article