Application of Bacillus cereus C1L for inducing systemic resistance to southern corn leaf blight
Date Issued
2007
Date
2007
Author(s)
Yang, Ken-Haow
DOI
zh-TW
Abstract
Biocontrol strain C1L of Bacillus cereus screened from the rhizosphere of Lilium formosanum could prevent the severe incidence of lily leaf blight. In this study, the green house and field biocontrol trials were performed by application of strain C1L to the rhizosphere of cron plants. The result showed that B. cereus C1L could reduce severe incidence of cron leaf blight; In addition, strain C1L could promote plant growth as shown by the increase of chlorophyll content, leaf width and length, and plant fresh and dry weight. Treatment of bacterial suspension of B. cereus C1L could reduce the level of hydrogen peroxide in the corn leaves. The reverse transcription-polymerase chain reaction analysis indicated that expression of superoxide dismutase gene (SOD4) and cytosolic ascorbate peroxidase gene (cAPX) increased slightly in the leaves of B. cereus C1L-treated cron plants as compared to that of the control. The expression of SOD4 and cAPX increased significantly after Cochliobolus heterostrophus attack; however, that exhibited lesser extent in the leaves from B. cereus C1L-treated cron plants. Thus, that B. cereus C1L increase the tolerance of cron plants to C. heterostrophus infection by strengthening the basal resistance is proposed, which may be related to the regulation of plant physiology. On the other hand, screening of Tn917ac1-directed mutants of B. cereus C1L for the reduction of ISR in maize againt C. heterostrophus achieved two ISR mutants, C1LM-163 and C1LM-179, in that of Tn917ac1 was located in a plasmid pC1L8 of B. cereus C1L.
Subjects
植物促生根圈細菌
臘狀芽孢桿菌
玉米
玉米葉枯病菌
生物防治
系統性誘導抗病
Plant growth-promoting rhizobacteria
Bacillus cereus
maize
Cochliobolus heterostrophus
biocontrol
induced systemic resistance
Type
other
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