Ror2 Signaling regulates Golgi structure and transport through IFT20 for tumor invasiveness
Journal
Scientific Reports
Journal Volume
7
Journal Issue
1
Date Issued
2017
Author(s)
Nishita M
Abstract
Signaling through the Ror2 receptor tyrosine kinase promotes invadopodia formation for tumor invasion. Here, we identify intraflagellar transport 20 (IFT20) as a new target of this signaling in tumors that lack primary cilia, and find that IFT20 mediates the ability of Ror2 signaling to induce the invasiveness of these tumors. We also find that IFT20 regulates the nucleation of Golgi-derived microtubules by affecting the GM130-AKAP450 complex, which promotes Golgi ribbon formation in achieving polarized secretion for cell migration and invasion. Furthermore, IFT20 promotes the efficiency of transport through the Golgi complex. These findings shed new insights into how Ror2 signaling promotes tumor invasiveness, and also advance the understanding of how Golgi structure and transport can be regulated. ? The Author(s) 2017.
Subjects
carrier protein; IFT20 protein, human; receptor tyrosine kinase like orphan receptor; small interfering RNA; cell motion; genetics; Golgi complex; human; mesenchymal stem cell; metabolism; microtubule; pathology; podosome; signal transduction; tumor cell line; tumor invasion; ultrastructure; Carrier Proteins; Cell Line, Tumor; Cell Movement; Golgi Apparatus; Humans; Mesenchymal Stem Cells; Microtubules; Neoplasm Invasiveness; Podosomes; Receptor Tyrosine Kinase-like Orphan Receptors; RNA, Small Interfering; Signal Transduction
Type
journal article