Phylogenetic Analysis of the Spike (S) Gene of the New Variants of Porcine Epidemic Diarrhoea Virus in Taiwan
Journal
Transboundary and Emerging Diseases
Journal Volume
64
Journal Issue
1
Pages
157-166
Date Issued
2017
Author(s)
Chiou H.-Y
Huang Y.-L
Deng M.-C
Chang C.-Y
Jeng C.-R
Tsai P.-S
Yang C
Pang V.F
Chang H.-W.
Chang, H.-W.
Chang H.-W.
Abstract
New variants of porcine epidemic diarrhoea virus (PEDV), which emerged in Taiwan in late 2013, have caused a high morbidity and mortality in neonatal piglets. To investigate the molecular characteristics of the spike (S) gene of the emerging Taiwan PEDV strains for a better understanding of the genetic diversity and relationship among the Taiwan new variants and the global PEDVs, full-length S genes of PEDVs from nine 1–7?day-old piglets from three pig farms in the central and southern Taiwan were sequenced and analysed. The result of phylogenetic analysis of the S gene showed that all the Taiwan PEDV strains were closely related to the non-S INDEL strains from US, Canada and China, suggesting a common ancestor for these strains. As compared with the historic PEDVs and CV777-based vaccine strains, the nine Taiwan PEDV variants shared almost the same genetic signatures as the global non-S INDEL strains, including a series of insertions, deletions and mutations in the amino terminal as well as identical mutations in the neutralizing epitopes of the S gene. The high similarity of the S protein among the Taiwan and the globally emerged non-S INDEL PEDV strains suggests that the Taiwan new variants may share similar pathogenesis and immunogenicity as the global outbreak variants. The development of a novel vaccine based on the Taiwan or the global non-S INDEL strains may be contributive to the control of the current global porcine epidemic diarrhoea outbreaks. ? 2015 Blackwell Verlag GmbH
Subjects
coronavirus spike glycoprotein; amino acid sequence; animal; chemistry; Coronavirus Infections; genetics; metabolism; phylogeny; physiology; pig; Porcine epidemic diarrhea virus; sequence alignment; Swine Diseases; Taiwan; veterinary; virology; Amino Acid Sequence; Animals; Coronavirus Infections; Phylogeny; Porcine epidemic diarrhea virus; Sequence Alignment; Spike Glycoprotein, Coronavirus; Swine; Swine Diseases; Taiwan
SDGs
Other Subjects
coronavirus spike glycoprotein; amino acid sequence; animal; chemistry; Coronavirus Infections; genetics; metabolism; phylogeny; physiology; pig; Porcine epidemic diarrhea virus; sequence alignment; Swine Diseases; Taiwan; veterinary; virology; Amino Acid Sequence; Animals; Coronavirus Infections; Phylogeny; Porcine epidemic diarrhea virus; Sequence Alignment; Spike Glycoprotein, Coronavirus; Swine; Swine Diseases; Taiwan
Type
journal article