Effects of low-intensity ultrasound (LIUS) on chondrogenic differentiation of synovial cells cultured on chitosan substrate
Date Issued
2012
Date
2012
Author(s)
Yoriki, Junichi
Abstract
Synovial membrane has been shown to contain mesenchymal stem cells. It is very important for stem cells to control cell-cell interaction for chondrogenic induction. Synovial cells cultured on TCPS exhibit normal fibroblast-like morphology. Cells formed spheroids when cultured on chitosan-coated substrate. Cells cultured on chitosan under low-intensity ultrasound (LIUS) stimulation formed spheroids larger than that on chitosan alone. Synovial cells cultured on TCPS could not undergo chondrogenic differentiation but cells cultured on chitosan differentiated into chondrocytes due to their spheroid formation as assessed by Safranin O staining. Furthermore, LIUS (0.1 W, 1MHz, duty cycle 10 %) applied every day enhanced more GAGs expression than cultured on chitosan only. In this study, the expression of chondrogenesis marker genes was observed. Gene expression of sox9 was increased by 1.8 and 2.7 folds by cells cultured on chitosan alone and chitosan with LIUS stimulation, respectively at early stage of cell culture. These results indicate that LIUS enhances sox9 mediated chondrogenic differentiation.
Subjects
low-intensity ultrasound (LIUS)
chitosan
synovial cells
TGF-beta 1
chondrogenic differentiation
Type
thesis
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