Nobiletin Modulates Adipocyte-Derived Exosomal miRNA to Improve Liver Lipid Metabolism in Obese Mice
Journal
Journal of Agricultural and Food Chemistry
Journal Volume
73
Journal Issue
35
Start Page
21959
End Page
21975
ISSN
0021-8561
1520-5118
Date Issued
2025-08-20
Author(s)
Abstract
This study examines the antiobesity properties of nobiletin through its influence on extracellular vesicles, particularly exosomes. Nob-ELVs (nobiletin with adipocyte-derived extracellular vesicles) significantly reduce fat accumulation in adipocytes without compromising cell viability. In vitro studies showed that introducing Nob-ELVs to 3T3-L1 preadipocytes led to marked reductions in fat accumulation. RNA sequencing indicated that Nobiletin alters the exosomal miRNA profile, downregulating miR-802-5p and others while upregulating miR-574-5p and related miRNAs. In a high-fat diet mouse model, Nob-ELVs prevented significant weight gain and improved food conversion efficiency. Mice receiving Nob-ELVs exhibited reduced hepatic lipid droplet accumulation and altered protein expressions, enhancing SIRT1 and FGF21 while suppressing ACC, FASN, and CD36. These findings suggest that Nob-ELVs inhibit fat accumulation and enhance lipid metabolism by modulating exosomal miRNAs, offering insights for new obesity treatment strategies.
Subjects
3T3-L1
adipocyte-derived extracellular vesicles
miRNAs
nobiletin
obesity
SDGs
Publisher
American Chemical Society (ACS)
Type
journal article