Isolation and identification of antitumor and anti-inflammatory active components from adlay bran
Date Issued
2008
Date
2008
Author(s)
Lee, Ming-Yi
Abstract
Adlay (Coix lachryma-jobi L. var. ma-yuen Stapf ) is an one year grass crop that has long been used as a traditional Chinese medicine to treat tumor and inflammation. Recent studies have continuously demonstrated the physiological effects of adlay and reveal that duhulled adlay posseses a higher anti-tumor and anti-inflammation activity than polished adlay. In summation of references, the adlay seeds contain some anti-tumor or anti-inflammation active compounds especially in the adlay bran. However, the active constituents involved in anti-tumor or anti-inflammation activity are still unknown. Therefore, the aim of our study is based on to discuss function of anti-tumor and anti-inflammation in food, to separate and identify the physiological effective constituents contained in adlay bran via active fractions.he study results show that the ethyl acetate fraction from adlay bran methanolic extract (ABM-EtOAc) had the highest ability to reduce survival rate of lung and colorectal carcinoma cell. It is also found that inhibition of cell cycle phase change, and cell apoptosis. Concerning anti-inflammation research, ABM-EtOAc and ABM-BuOH subfractions all inhibit LPS-induced TNF-α and IL-1β which released from RAW 264.7. Furthermore, ABM-EtOAc and ABM-BuOH subfractions inhibit COX-2 expression of A549 and HT29 at 50 μg/ml concentration, while constitutive COX-1 expression is not affected. Moreover, the ABM-BuOH subfraction is the best. Thus, these results indicate that ABM-EtOAc and ABM-BuOH subfractions must have some anti-tumor or anti-inflammation active compounds. They inhibit cancer cell growth by promoting apoptosis of cancer cell. The chemical compound of plant has preventative effects on chronic inflammation-related cancer. urthermore, 25 pure compounds are isolated and identified by inhibiting growth of lung cancer and colon cancer. The coixspirolactam A, coixspirolactam B, and coixspirolactam C are newly identified compounds. Caffeic acid, protocatechuic acid, chlorogenic acid, 3-O-coumaroyl-5β-sitostanol, 3-O-caffeoyl-5β-sitostanol, methyl dioxindole-3-acetate and 24,25-oxidocycloartanol are first isolated from adlay. From the anti-tumor activity test, it shows that there are only Caffeic acid, Protocatechuic acid, and Chlorogenic acid have anti-tumor activity in phenolic acid. rom another hand, via anti-inflammation activity, the nine pure compounds, which are not found in ABM-EtOAc, are isolated from ABM-BuOH using many different kinds of isolation technology. Including one Apocarotene: Loliolide, one triterpinoid: 3β-Hydroxyolean-18-en-28-oic acid, one lignan: Pinoresinol, one phenolic ester: (E)-methyl 3,4-dihydroxycinnamate, one sugar-link sterol: β-Sitosteryl-3β-glucopyranoside, one isoflavone: Biochanin A, three flavonoid: 5, 7-Dimethoxy-4''-hydroxyflavan, 4'', 5, 7-Trihydroxyflavone (apigenin), Farrerol, all of the nine pure compounds are first isolated from adlay.
Subjects
adlay bran
lung cancer
colorectal carcinoma
apoptosis
anti-tumor
anti-inflammation
active compound
SDGs
Type
thesis
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