Synthesis of Hollow Mesoporous Hydroxyapatite Nanoparticles for Intracellular Drug Delivery
Date Issued
2011
Date
2011
Author(s)
Yang, Ya-Heui
Abstract
This study reports the synthesis and intracellular drug delivery of hollow mesoporous hydroxyapatite nanoparticles (hm-HANPs). For the synthesis part, the effects of several critical factors including the amount of ethylene glycol (EG), reaction time to form CaCO3 cores, concentrations of Ca(CH3COO)2(aq) and NaHCO3(aq), concentration of H3PO4(aq) and the amount of CH3COOH(aq) were investigated for synthesizing hollow mesoporous hydroxyapatite nanoparticles (hm-HANPs). We optimized the reaction conditions as follows: The ratio of CaCO3 precursor solution to EG was 1:5. Reaction time to form CaCO3 cores was 3hr. The CaCO3 precursor solution was 0.3 M Ca(CH3COO)2(aq):0.3 M NaHCO3(aq). The concentrations of H3PO4(aq) was 0.01 M. And the ratio of the CaCO3 precursor solution to CH3COOH(aq) was over 1:3.125. The monodispersed hm-HANPs with a defined particle size (400 x 600 nm) could be achieved. For the intracellular drug delivery part, doxorubicin (DOX) was used as an anticancer drug and loaded into hm-HANPs. We found that hm-HANP exhibited higher (i.e., five times) drug loading capacity than HANPs without hollow core. In addition, mesoporous shell of hm-HANPs slowed down the release of loaded DOX, reducing the burst release at the beginning. Our hm-HANPs also exhibited pH-responsive release behavior. Compared with free DOX, the anticancer efficacy of DOX-loaded hm-HANPs was greatly enhanced. This pH-sensitive property of DOX-loaded, hm-HANPs would be useful for controlled drug delivery system.
Subjects
hydroxyapatite
hollow
nanoparticles
BT-20 cancer cells
drug carrier
drug delivery
SDGs
Type
thesis
File(s)![Thumbnail Image]()
Loading...
Name
ntu-100-R98524017-1.pdf
Size
23.54 KB
Format
Adobe PDF
Checksum
(MD5):8f750960a305877b3fc9459b3f679654