The anti-tumor effect of flavonoids on Du145 parental cells and highly invasive potential Du145-III.

The flavonoids, which are primary benzo-γ- pyrone ( phenylchromone) derivatives, comprise a massive group of polyphenolic compounds. The immensely diverse group broadly comprises distinct classes such as flavonols, flavans and proanthocyanidin, anthocyanidinsm flavanones, flavones, isoflavones and neoflavonoids. They are universally present in fruits, cereals, legumes, vegetables, nuts, seeds, herbs, spices, stems, flowers as well as in beverages such as tea, cocoa, beer and wine. As components of edible plants and plant foodstuffs they constiturte an integral part of the human diet. The documented biological effects of dietary flavonoids include anti-flammatory, antiallergic antimicrobial, hepatoprotective, antiviral, antithrombotic, cardioprotective, capillary strengthening, antidiabetic, anticarcinogenic and antineoplastic effects, among others.
In this thesis, we established the relatively higher invasive potential tumor cell line: Du145-I, II and III from Du145-P (parental) using Boyden chamber invasion assay. The Du145-III secreted higher amount of MMPs, higher migration and angiogenic ability as compared to Du145-P. According to the amount of MMPs secretion, migration and angiogenic ability, we concluded that Du145-III had relative higher invasive potential than that of Du145-P. Luteolin and Quercetin were documented as protein kinase inhibitor, they inhibit the activity of protein kinase by blocking the ATP binding site in catalytic unit. In this study, I tested the anti-tumor effect of Luteolin and Quercetin on Du145-P and Du145-III cells. Luteolin and Quercetin efficiently inhibit the growth of Du145-P and Du145-III cells. In addition, Luteolin and Quercetin both exert the inhibitory effect of MMPs secretion, migration and angiogenesis of Du145-P and Du145-III cells.
In a separate study, using PC-3 prostate tumor cell line, we observed that the profile of MMPs secretion is similar to that of Du145. Therefore, we intend to investigate the profile of other prostate tumor cell lines, including 22RV1 and LNCAP, in order to confirm those MMPs are organ specific.
We also established the prostate tumor cell lines Du145-P and Du145-III that could excite the red and green fluorescence. In the future, we intend to establish the animal model to observe the metastasis pathway of prostate tumor cell line by using the Du145-P and Du145-III that could excite the red and green fluorescence.