Anti-tumor and Immunomodulation of Polypeptide Fraction from Jelly-fig (Ficus awkeotsang Makino) Achenes
Date Issued
2005
Date
2005
Author(s)
Chang, Jia-Huei
DOI
zh-TW
Abstract
Polypeptide fraction (PF) from jelly-fig (Ficus awkeotsang Makino) achenes was prepared to determine its effect on proliferation, differentiation, and apoptosis of human leukemic cells (U937) in vitro and immunomodulation and antitumor of CT26 in vivo. First, human peripheral blood mononuclear cells were stimulated by PF (100 μg/mL) for 1 day to prepared conditioned media (PF-MNC-CM-1). With which high growth inhibition (94%) on U937 cells and nitroblue tetrazolium (NBT) positive percentage, nonspecific esterase (NSE) positive percentage and surface antigen expressions of differentiated cells were observed. By morphological examination, U937 cells were confirmed to be differentiated into mature monocytes or/and macrophages. Through an ELISA, we found that MNC-CM-1 (stimulated by 100 μg/mL PF) contained high levels of IL-1β (4446.5±58.5 pg/mL), TNF-
Subjects
誘導分化
細胞凋亡
愛玉瘦果
多胜肽
區分
單核細胞條件培養液
differentiation induction
apoptosis
MNC-CNs
Jelly-fig
Polypeptide Fraction
Type
thesis