Studies on the chemical constituents of the fruit of Cnidium monnieri (L.) Cusson

Cnidium monnieri (L.) Cusson distributes over Taiwan and mainland China. The dried fruit of Cnidium monnieri (L.) Cusson is an efficient medicine for several diseases.
The methanol extract from the dried fruit of Cnidium monnieri (L.) Cusson was concentrated to give a residue which was partitioned between ethyl acetate and water. The ethyl acetate layer was separated by column chromatography on silica gel, preparative thin layer chromatography and high performance liquid chromatography (HPLC) to give 45 compounds. Of these compounds, 10 news and 3 isolated for the first time from natural sources are elucidated. The total isolated included 29 coumarins, 7 chromones, 2 aromatic compounds, 3 monoterpenoids, 1 sesquiterpenoid, 1 diterpenoid and 2 steroids.
The known compounds were identified by spectral analysis and compared with those reported in literatures or authentic samples. The structures of new compounds were elucidated by spectral evidence including 1D and 2D techniques.