行政院國家科學委員會專題研究計畫期中進度報告:家禽白血病J亞群病毒基因、抗原性及病原性之演變(2/3)
Date Issued
2003
Date
2003
Author(s)
王金和
DOI
912313B002372
Abstract
Avian luekosis virus causes various
tumor and immunosuppreion in chickens
and result in great economical losses
in poultry industry, especial subgroup
J. This disease has occurred worldwide
including Taiwan. In order to
understand the serological profile
after J virus infection, the chickens
in the three grand parent stocks in
Taiwan were collected for antigen and
antibody detection by ELISA. The blood
samples were collected at the week ages
of 1, 6, 16, 26 and 46. The antigen
positive rates in Farm A are: A line-
17.39, 12.12, 5.8, 10.14%; B line - 8.7,
14.93, 14.49, 10.14%; C line- 30.43,
25.53, 10.14, 23.19%; D line- 60.87,
42.42, 17.39, 26.09%. In Farm B: A line -
20, 25, 13.04, 15.94%; B line- 20, 30,
13.04, 13.04%; C line- 28.57, 23.33,
13.04, 14.49%; D line- 33.33, 28.33,
44.93, 31.88%. In Farm C: Male- 2.17,
17.39, 10, 10.87%; Female- 43.48, 11.96,
11.43, and 38%. The infected flocks
were confirmed by RT-PCR with specific
primers and gene sequencing. The
results showed that the p27 antigen
increased from 1 week old to 6 weeks old.
However, the uninfected flocks showed
the inverse. The p27 antigen decreased
from one week old to 6 weeks old.
Furthermore J virus infection profile
was detected in DF1 cell by flow
cytometry.
Subjects
Avian leucosis virus
serology
flow cytometry
Publisher
臺北市：國立臺灣大學獸醫學系暨研究所
Type
report
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