The Inducible Transposon System for Rice Functional Genomics
Journal
Botanical Studies
Journal Volume
48
Journal Issue
1
Start Page
1
End Page
11
ISSN
1817-406X
Date Issued
2007-01
Author(s)
吳紀德(Gideon Wu)
謝嘉珊(Chia-Shan Hsieh)
莊函寧(Han-Ning Chuan)
黃紀穎(Ji-Ying Huang)
葉凌君(Ling-Chun Yeh)
謝亞雄(Ya-Hsiung Shieh)
杜鎮(Jenn Tu)
Abstract
The one-component inducible transposon system for rice functional genomic studies was
assessed. In contrast to the native Ac transposon, INAc contains the transposase gene under the control of the
inducible promoter (PR-1a) from tobacco. To examine whether INAc can be used in cereals, the behavior of
INAc was analyzed with transgenic rice plants containing a single copy of the INAc element. Treatment of
rice calli with salicylic acid induced transposition of INAc in somatic tissue, and the transposition efficiency
of INAc was dose dependent. Furthermore, a high throughput method for detection of new transposed INAc
was developed. Analyzing the flanking sequences of the transposed INAc indicated the independent insertions.
Given the fact that a number of different types of Ac/Ds vectors have been already examined in rice, the
importance of a “controlled” transposon system to yield knockout mutants or new transgenic plants was
discussed.
Subjects
Ac transposase
Inducible promoter
Salicylic acid
Transposon tagging
Publisher
Springer
Type
journal article