The Effects of High-fat Diets Containing White Fruits and Vegetable on Gene Expression of Colonic Mucosa in ICR Mice
Date Issued
2011
Date
2011
Author(s)
Wu, Shiau-Ling
Abstract
Colorectal cancer (CRC) has become especially prevalent in developed countries. CRC has the highest incidence since 2006 in Taiwan and now is the 3rd leading cause of cancer death. Study evidence has suggested a close association between development of CRC and dietary patterns. To reduce the risk, dietary recommendations emphasize that low-fat and high-fruits and vegetables diet. In general, fruits and vegetables contain abundant mixture of disease-preventing substances including vitamins, minerals, dietary fiber and phytochemicals and the majority of epidemiological evidence supporting the high-fruits and vegetables diet is related to decreased risk of CRC. In recent years, human dietary pattern will incline to be more westernized, especially higher intake of fat. Therefore, we investigated the effect high-fat diets containing white fruits and vegetable on gene expression of colonic mucosa in ICR mice using DNA microarray technology. Male ICR mice of outbred strain were purchased from BioLASCO Taiwan Co., Ltd.
They were randomized by body weight into six experiment groups：a high-fat 93G (HF), a high-fat litchi group (HFL), a high-fat longan group (HFO), a high-fat guava group (HFG), a high-fat wax gourd group (HFW), and chow diet (Chow). A high fat basal diet (14.4% oil, 30 % Kcal from fat) was modified from AIN-93G. Fruits and vegetable experimental diets were prepared by adding lyophilized food powder to basal diet at 10% wt and adjusting the macronutrient contents to reach similar energy density.After feeding for four weeks, mice were killed by CO2 asphyxiation. Total RNA of colonic mucosa was extracted from each mice and pooled RNA sample corresponding to experiment group were subjected to Agilent Mouse Whole Genome Oligo Microarray assay using One-color Microarray hybridization protocol. Then, Q-PCR was used to validate the microarray results. The liver and serum was collected for analysis of triglyceride, cholesterol and vitamin C. The W group had lower weight gain and liver triglyceride level than HF93G and high-fat fruits groups. However, daily food intakes, estimated energy intake showed no significant difference among the HF93G and high-fat fruits and vegetable groups. Gene expression were increased in HF93G compared to Chow group, including related function to immune response, oxidative stress response, cell proliferation, cell apoptosis, and lipid metabolism. Gene expression were decreased in high-fat fruits and vegetable groups compared to HF93G group, including related function to immune response, oxidative stress response, cell proliferation, cell apoptosis, and lipid metabolism. Cyclooxygenase-2 (COX-2) and ki67 gene expression of individual mice was measured by Q-PCR and showed HF93G group had higher than Chow and high-fat fruits and vegetable groups. Collectively, these data suggest that consumption of a high-fat diet interferes with biological function involving colonic immune response, oxidative stress response, cell proliferation, cell apoptosis, and lipid metabolism, and may exert a proinflammatory stimulus in the colon.The white fruits and vegetables may be beneficial in decreasing adverse effect of high-fat diet.
Subjects
colon
high fat diet
litchi
longan
guava
wax gourd
microarrays
ICR mice
SDGs
Type
thesis
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