Separation of subunits of pike eel gonadotropin by hydrophobic interaction chromatography
Resource
Journal of Chromatography 215: 229-233
Journal
Journal of Chromatography 215
Pages
229-233
Date Issued
1981
Date
1981
Author(s)
Abstract
Subunits of pike eel gonadotropin were dissociated in propionic acid and separated by hydrophobic interaction chromatography on phenyl-Sepharose CL-4B with a high yield (79%). Isolated subunits were homogeneous, as tested by sodium dodecyl sulphate disc electrophoresis and by N-terminal analysis. The biological activities of two subunits, S-I and S-II, were 0% and 2.8% of native molecule, respectively, as assayed by stimulation of androgen production on carp testes. Reassociated molecules restored about 75% activity.
SDGs
Type
journal article
File(s)![Thumbnail Image]()
Loading...
Name
05.pdf
Size
319.02 KB
Format
Adobe PDF
Checksum
(MD5):840d2cb16e0dc6e22761e6ee88aeaa48