NF-κB p50 promotes tumor cell invasion through negative regulation of invasion suppressor gene CRMP-1 in human lung adenocarcinoma cells
Journal
Biochemical and Biophysical Research Communications
Journal Volume
376
Journal Issue
2
Pages
283-287
Date Issued
2008
Author(s)
Abstract
Lung adenocarcinoma Cl1-5 cells were selected from parental Cl1-0 cells based on their high metastatic potential. In a previous study, CRMP-1, an invasion suppressor gene, was shown to be suppressed in Cl1-5 cells. However, the regulation of CRMP-1 expression has not been explored. In this study, we showed nuclear factor-κB controls CRMP-1 expression. The electromobility shift assay showed that while Cl1-0 cells exhibited low NF-κB activity in response to TNF-α, an abundance of basal and TNF-α-induced NF-κB-DNA complex was detected in Cl1-5 cells. Supershift-coupled EMSA and Western blotting of nuclear proteins, however, revealed p50 protein, but not classic p65/p50 heterodimer in the complex. ChIP and EMSA demonstrated that p50 binds to a κB site residing between -1753 and -1743 of the CRMP-1 promoter region. Transfection of antisense p50 gene into Cl1-5 cells increased the CRMP-1 protein level and decreased the invasive activity of Cl1-5 cells. ? 2008 Elsevier Inc. All rights reserved.
SDGs
Other Subjects
immunoglobulin enhancer binding protein; protein p50; synaptotagmin I; tumor necrosis factor alpha; CRMP1 protein, human; immunoglobulin enhancer binding protein; nerve protein; transcription factor RelA; antisense p50 gene; article; cancer invasion; cell selection; controlled study; CRMP 1 gene; DNA protein complex; gel mobility shift assay; gene; gene expression regulation; gene overexpression; genetic transfection; human; human cell; lung adenocarcinoma; metastasis potential; nucleotide sequence; priority journal; promoter region; tumor suppressor gene; Western blotting; adenocarcinoma; cancer invasion; gene expression regulation; genetics; lung tumor; metabolism; pathology; tumor cell line; Adenocarcinoma; Cell Line, Tumor; Electrophoretic Mobility Shift Assay; Gene Expression Regulation, Neoplastic; Genes, Tumor Suppressor; Humans; Lung Neoplasms; Neoplasm Invasiveness; Nerve Tissue Proteins; NF-kappa B p50 Subunit; Promoter Regions (Genetics); Transcription Factor RelA; Tumor Necrosis Factor-alpha
Type
journal article