The Inhibitory Effect of Panax notoginseng Fermented with Lactic Acid Bacteria on the Growth of Hepatoma Cells
Date Issued
2005
Date
2005
Author(s)
Mou, Yu-Chen
DOI
zh-TW
Abstract
Panax notoginseng (Burk.) F. H. Chen, one of the most famous traditional Chinese medicine (TCM), possesses anticarcinogenic activity. The major active components had been identified as saponins.
The aims of this research were to enhance the antihepatoma activity of P. notoginseng by lactic acid bacteria fermentation, to investigate the bioactive components in the fermentation products which are responsible for the antihepatoma activity, and to study the possible mechanism of the inhibitory effect of the fermentation products on hepatoma cell lines.
According to the results of static fermentation, the basic fermentation conditions, including species and co-culture of lactic acid bacteria, and the method of pretreatment of the medium were decided. It was found that inoculation of 8 species of lactic acid bacteria and co-cultured with P. notoginseng for 24 hours could enhance the antitumor activity of the herb. When the fermentation was carried out in 6.6 liter bioreactor, the fermentation broth with the stronger anti-hepatoma activity could be produced.
The changes of saponins in the fermentation broths were analyzed by HPLC, and a negative correlation between the viability of hepatoma cells and 5 peaks located in the lower polar region on the HPLC profile were observed.
It was also found that the possible mechanism of the inhibitory effect was to arrest the cell cycle at G0/G1 phase. The cytotoxic effects on Hep G2 and Hep 3B cells were higher than those on rat primary hepatocytes.
Subjects
三七
發酵
肝癌細胞
Panax notoginseng
fermentation
hepatoma cells
Type
thesis
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