Development of CMOS gratings for spectrometer in Real-Time PCR
Date Issued
2006
Date
2006
Author(s)
Jian, Huang-Cing
DOI
zh-TW
Abstract
First, according to the structure and need of the optics examination system in Real-Time PCR and the CMOS manufacturing process technique, this study go to develop the parameter and shape of grating. The size of CMOS grating designed is 2 mm × 2 mm. Second, the simulation imitates the light road of optics experiment equip by light tracing imitating software TracePro. Third, the experiments measure the fluorescence spectrum of the DNA sample. Finally, in order to inquire into the function and the benefit of the CMOS grating, the results compare the experiment of the CMOS grating with the commercial grating.
Test the result manifestation, the CMOS grating can measures the fluorescence of DNA sample and this research makes use of the CMOS grating that to be used particularly for the Real-Time PCR optics examination system successfully. Besides, the area of CMOS grating can reduce 80% comparing with the commercial grating and the cost of CMOS grating can reduce 21% comparing with commercial. To design the optics examination system in Real-Time PCR, this thesis provided another direction of manufacturing process and parameter to is the reference that the future improves.
Subjects
DNA樣本試劑螢光量測
CMOS
基因定量分析
光柵
grating
REAL-TIME PCR
Type
thesis
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