E2F4下游基因於抑制腫瘤侵入之鑑定
Identification of E2F4 Downstream Genes Involved in Tumor Invasion
Date Issued
2004
Date
2004
Author(s)
Chen, Mei-Ying
DOI
en-US
Abstract
Abstract
E2F4, a member of the E2F family of transcription factors, is abundant in non-proliferating and differentiated cells where it plays an important role in the suppression of proliferating associated genes. Several lines of evidence indicate that E2F1 is involved in neoplastic development, while little is known about E2F4 in tumor formation. Based on our previous study, the expression level of E2F1 was found not significantly associated with the progressiveness of more invasive sublines, while E2F4 showed progressively decreased. Stable clones overexpressing E2F1 in HeLa showed no significantly enhanced migration and invasion potential. However, when overexpressing E2F4, the tumor cells were found with reduced invasion potentials. In order to identify the down stream gene involved in the inhibition of tumor invasion in E2F4, Microarray analysis has been adapted to detect differentially expressed in Hela/E2F4.7 and Hela/PCINEO. The up-regulation of UCHL1 and DAB2 and the down-regulation of P53, KRT8, CTGF and MDK were observed. The differential expression of genes in Hela/E2F4.7 and Hela/PCINEO were confirmed by Semi-Quantitative RT-PCR analysis. The up-regulated genes are both cell cycle related, DAB was a tumor suppressor gene. The down-regulated genes are either tumor invasion, metastasis or angiogenesis associated. The potential roles of E2F4 expression in tumor metastasis were discussed.
Subjects
E2F4
生物晶片
腫瘤轉移及入侵
tumor invasion
microarray
Type
thesis
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