Lutein production by microalgae using corn starch wastewater pretreated with rapid enzymatic hydrolysis
Journal
Bioresource Technology
Journal Volume
352
Date Issued
2022
Author(s)
Abstract
The main purpose of this study was to explore the pretreatment process of corn starch wastewater (CSW) and engineered microalgae cultivation strategy to improve the nutrient recovery from wastewater and the yield of microalgae lutein. One-stage enzymatic hydrolysis utilizing α-amylase and glucoamylase simultaneously was established to efficiently harvest a maximum concentration of reducing sugar content of 7.26 g/L from CSW in 50 min. Lutein yield of 10.96 mg/L was obtained under 24 h continuous illumination with 2200 Lux light intensity. Furthermore, a cyclic feeding cultivation strategy was developed to improve lutein accumulation and COD removal up to 25.9 mg/L and 50.7%, respectively, after three cultivation cycles. Lutein yield of 14.86 mg/L and COD removal efficiency of 73.2% was achieved with further implementation in actual wastewater. This work provided a new perspective in developing the potential of cultivating microalgae with corn starch wastewater to produce high-value lutein. © 2022 Elsevier Ltd
Subjects
Circular Economy; Enzymatic Pretreatment; Lutein; Microalgae; Starch Wastewater
SDGs
Other Subjects
Enzymatic hydrolysis; Microorganisms; Starch; Circular economy; Corn starch; Enzymatic pretreatment; Glucoamylase; Lutein; Maximum concentrations; Microalgae cultivation; Nutrient recovery; Pretreatment process; Starch wastewater; Microalgae; amylase; glucan 1,4 alpha glucosidase; nitrogen; organic matter; phosphorus; starch; xanthophyll; starch; xanthophyll; chemical oxygen demand; enzyme; enzyme activity; hydrolysis; light intensity; microalga; wastewater; wastewater treatment; algal cell culture; algal growth; Article; biomass production; bioremediation; biosynthesis; chemical oxygen demand; Chlorella sorokiniana; concentration (parameter); controlled study; energy consumption; enzymatic hydrolysis; filtration efficiency; growth rate; illumination; light intensity; maximum concentration; microbial biomass; nitrogen concentration; nonhuman; nutrient cycling; pH; photoperiodicity; quantum yield; reaction analysis; reaction duration (chemistry); reaction optimization; reaction temperature; recycling; surface area; waste component removal; waste water management; water temperature; biomass; hydrolysis; maize; microalga; wastewater; Biomass; Hydrolysis; Lutein; Microalgae; Starch; Waste Water; Zea mays
Type
journal article