Study of Susceptibility of Mouse Pulmonary Stem/Progenitor Cells to Influenza Virus and Human Herpes Simplex Virus Type 1 Infections
Date Issued
2009
Date
2009
Author(s)
Tsou, Yu-Tien
Abstract
Stem cells are characterized of self-renewal and multipotent differentiation, have been implicated to repair the injured organ and tissue. In 2006, Ling et al. established a primary culture system to generate mouse pulmonary Oct-4+ stem/progenitor cells, which can support SARS-CoV infection. In 2007, Chen et al. further reported the colocalization of Oct-4 and SARS viral protein in the SARS-infected cells in lung autopsy of SARS-infected patients.n this study, we aimed at determining the susceptibility of mouse pulmonary stem/progenitor cells to influenza virus and herpes simplex virus type 1(HSV-1), and study the mechanisms of virus infection. First, cytopathic effects were immediately observed in influenza virus infected stem/progenitor cells at 24hr. The expression of in stem cell colony and the gradual increase of release infectious virus particles in the supernatant, indicated that the stem cells died support the replicate of influenza virus. The susceptibility of influenza virus to Oseltamivir in stem cells is not as significant as those observed in MDCK cell lines. Next, we found that stem cells are more sensitive to HSV-1 infect than HEp2 cell lines and cytopathic effects can be observed at 16hr post infection. The efficient replication of HSV-1 in stem cells is likely due to more efficient viral entry and DNA replication. he pulmonary stem/progenitor cells can be infected with influenza virus and HSV-1. The influences of viral infection in pulmonary stem/progenitor cells, such as repair of injured lung, require further analysis.
Subjects
pulmonary stem/progenitor cells
susceptibility
influenza virus
herpes simplex virus
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