Lack of a Y-chromosomal complement in the majority of gestational trophoblastic neoplasms
Journal
Journal of Oncology
Journal Volume
2010
Pages
364508
Date Issued
2010
Author(s)
Abstract
Gestational trophoblastic neoplasms (GTNs) are a rare group of neoplastic diseases composed of choriocarcinomas, placental site trophoblastic tumors (PSTTs) and epithelioid trophoblastic tumors (ETTs). Since these tumors are derivatives of fetal trophoblastic tissue, approximately 50% of GTN cases are expected to originate from a male conceptus and carry a Y-chromosomal complement according to a balanced sex ratio. To investigate this hypothesis, we carried out a comprehensive analysis by genotyping a relatively large sample size of 51 GTN cases using three independent sex chromosome genetic markers; Amelogenin, Protein Kinase and Zinc Finger have X and Y homologues that are distinguishable by their PCR product size. We found that all cases contained the X-chromosomal complement while only five (10%) of 51 tumors harbored the Y-chromosomal complement. Specifically, Y-chromosomal signals were detected in one (5%) of 19 choriocarcinomas, one (7%) of 15 PSTTs and three (18%) of 17 ETTs. The histopathological features of those with a Y-chromosome were similar to those without. Our results demonstrate the presence of a Y-chromosomal complement in GTNs, albeit a low 10% of cases. This shortfall of Y-chromosomal complements in GTNs may reinforce the notion that the majority of GTNs are derived from previous molar gestations. Copyright ? 2010 Kai Lee Yap et al.
SDGs
Other Subjects
amelogenin; protein kinase; zinc finger protein; Article; choriocarcinoma; female; genetic marker; genotype; histopathology; human; human tissue; polymerase chain reaction; syncytiotrophoblast cell; trophoblast; trophoblastic tumor; X chromosome; Y chromosome
Publisher
Hindawi Limited
Type
journal article