Investigating the Mechanistic Differences of Obesity-Inducing Lactobacillus kefiranofaciens M1 and Anti-obesity Lactobacillus mali APS1 by Microbolomics and Metabolomics
Journal
Frontiers in Microbiology
Journal Volume
11
Date Issued
2020
Author(s)
Abstract
Many studies have investigated the anti-obesity effects of probiotics in animal models and humans. However, few studies have focused on the mechanisms of obesity-inducing probiotics. In a previous study, we demonstrated that specific bacterial strains isolated from kefir, Lactobacillus kefirnofaciens M1 and Lactobacillus mali APS1, possess obesity and anti-obesity effects, respectively, in high-fat diet (HFD)-induced obese mice. Thus, in the present study, we systematically investigated whether APS1 and M1 affect energy homeostasis and lipid metabolism in HFD-induced obese mice and how this might be achieved. We observed that the M1/APS1 intervention influenced fat accumulation by regulating adipogenesis and inflammation-related marker expression both in vitro and in a HFD induced C57BL/6J mice model. We also observed putative links between key taxa and possible metabolic processes of the gut microbiota. Notably, families Christensenellaceae and S24_7 were negatively correlated with body weight gain through increase in the essential esterized carnitine for energy expenditure. These results suggest the importance of specific probiotic interventions affecting leanness and obesity of subjects under a HFD, which are operated by modulating the tripartite relationship among the host, microbiota, and metabolites. ? Copyright ? 2020 Lin, Chen, Li and Chen.
Subjects
Lactobacillus kefiranofaciens M1; Lactobacillus mali APS1; metabolomics; microbolomics; obesity
SDGs
Other Subjects
3 hydroxy 3 methylglutaric acid; ambion; CD68 antigen; corticosterone; fatty acid synthase; formaldehyde; gastric inhibitory polypeptide; glycoprotein p 15095; isoflurane; isoleucine; leptin; pancreas polypeptide; penicillin derivative; phosphate buffered saline; phosphatidylcholine; polysorbate 20; probiotic agent; propionyl carnitine; ribonuclease inhibitor; short chain fatty acid; streptomycin; sulfuric acid; unclassified drug; acidification; adipose tissue; animal experiment; animal model; Article; Bacteroides; Bifidobacterium; bioinformatics; body weight; cell differentiation; controlled study; DNA extraction; electrospray; food intake; inflammation; intestine flora; Lactobacillus; lactobacillus kefiranofaciens; lactobacillus mali aps1; lipid diet; lipid metabolism; lipid peroxidation; lipid storage; liquid chromatography-mass spectrometry; male; metabolomics; microscopy; mouse; nonhuman; obesity; oxidative stress; polyacrylamide gel electrophoresis; protein expression; protein phosphorylation; RAW 264.7 cell line; RNA extraction; time of flight mass spectrometry; transwell assay; ultracentrifugation
Type
journal article