以表現基因庫系統性選殖台灣克雷伯氏肺炎桿菌對乙內醯胺類藥物的抗藥性基因

Klebsiella pneumoniae is an important
pathogen inherently resists to ampicllin and
sometimes other beta-lactams. Through
constructing λ -ZAP II phage expression
libraries of K. pneumoniae genome and screening beta-lactam-resistant transformants,
we found SHV-1a （14/24 ）andSHV-1 （7/24 ）
are the most common genetic basis of
ampicillin resistance in community-acquired
K. pneumoniae strains in Taiwan. There is no
significant difference in the beta-lactamase
genotypes between strains causing primary
liver abscess （n=12 ）and strains did not
cause primary liver abscess （n=12 ）.
SHV-27 （1/24 ）, SHV-41 （1/24 ）and
TEM-116 （24/24 ）, which have been reported
as encoding extended-spectrum beta-
lactamase (ESBL) but not confirmed in the
present study, were also identified in some
community-acquired strains. In contrast,
TEM-1b （23/48 ）and SHV-12 （16/48 ）are
the predominant ESBL genotypes in
nosocomial strains, followed by CTX-M3
（10/48 ）. Nine ESBL strains did not harbor
known SHV, TEM or CTX-M genotypes.