Effect of hyperoxia on retinoid metabolism and retinoid receptor expression in the lungs of newborn mice
Journal
PLoS ONE
Journal Volume
10
Journal Issue
10
Pages
e0140343
Date Issued
2015
Author(s)
Chen H.-J.
Abstract
Background Preterm newborns that receive oxygen therapy often develop bronchopulmonary dysplasia (BPD), which is abnormal lung development characterized by impaired alveologenesis. Oxygen-mediated injury is thought to disrupt normal lung growth and development. However, the mechanism of hyperoxia-induced BPD has not been extensively investigated. We established a neonatal mouse model to investigate the effects of normobaric hyperoxia on retinoid metabolism and retinoid receptor expression. Methods Newborn mice were exposed to hyperoxic or normoxic conditions for 15 days. The concentration of retinol and retinyl palmitate in the lung was measured by HPLC to gauge retinoid metabolism. Retinoid receptor mRNA levels were assessed by real-time PCR. Proliferation and retinoid receptor expression in A549 cells were assessed in the presence and absence of exogenous vitamin A. Results Hyperoxia significantly reduced the body and lung weight of neonatal mice. Hyperoxia also downregulated expression of RARα, RAR?, and RXR? in the lungs of neonatal mice. In vitro, hyperoxia inhibited proliferation and expression of retinoid receptors in A549 cells. Conclusion Hyperoxia disrupted retinoid receptor expression in neonatal mice. ? 2015 Chen, Chiang.2015 Chen, Chiang. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
SDGs
Other Subjects
retinoic acid receptor; retinoic acid receptor alpha; retinoic acid receptor gamma; retinoid; retinoid X receptor gamma; retinol; retinol palmitate; retinoic acid receptor; retinoid; retinol; thymidine; A549 cell line; animal cell; animal tissue; Article; body weight; cell proliferation; controlled study; down regulation; female; growth inhibition; high performance liquid chromatography; human; human cell; hyperoxia; immunohistochemistry; in vitro study; liver; lung; lung development; lung weight; mouse; newborn; nonhuman; protein analysis; protein expression; protein localization; protein metabolism; real time polymerase chain reaction; survival rate; tissue level; analogs and derivatives; animal; drug effects; hyperoxia; Institute for Cancer Research mouse; metabolism; pathology; survival analysis; tumor cell line; Animals; Animals, Newborn; Cell Line, Tumor; Cell Proliferation; Humans; Hyperoxia; Liver; Lung; Mice, Inbred ICR; Receptors, Retinoic Acid; Retinoids; Survival Analysis; Thymidine; Vitamin A
Publisher
Public Library of Science
Type
journal article