One-Stage Cultivation of Haematococcus pluvialis under Stressful Conditions
Date Issued
2009
Date
2009
Author(s)
Chen, Guan-Yan
Abstract
Haematococcus pluvialis is the most important source of natural astaxanthin which has the ability of antioxidative activity higher than β-carotene and vitamin E. Astaxanthin is a high-value-added product. Currently, the culture method of commercial Haematococcus pluvialis for producing astaxanthin is a two – stage mode. n this study, one-stage cultivation of Haematococcus pluvialis using stressful conditions: high light intensity (150 μEm-2s-1) and high CO2 concentration (10%) was developed to increase cell density from 0.02 to 1.07 g/L after 18-day culture. The amount of extracted astaxanthin was 4.482 mg. By contrast, the astaxatnthin extract amount 0.775 mg by a conventional two-stage culture which had initial cell density grew from 0.02 to 0.56 g/L under atmospheric CO2 concentration and low light intensity (25 μEm-2s-1) for 18 days and then switch to a second stage with high light intensity 300 μEm-2s-1 for 7 days to induce the accumulation of astaxanthin. When CO2 concentration elevated to 15% and light intensity increased to 200 μEm-2s-1, cell density increased from 0.2 g/L to 1.13 g/L and the obtained astaxanthin was 3.410 mg. ur results demonstrate that one-stage cultivation of Haematococcus pluvialis under stressful conditions for the production of high yield of astaxanthin is innovative approach compared to the conventional two-stage culture method. In addition, the high concentration of 15% CO2, discharged CO2 concentration from power plant, employed for the one-stage microalgae culture can be sequestered to a lower emission concentration for alleviate global warming effect.
Subjects
CO2
Haematococcus pluvialis
photoinhibition
astaxanthin
One-stage culture
Type
thesis
File(s)![Thumbnail Image]()
Loading...
Name
ntu-98-R96524058-1.pdf
Size
23.53 KB
Format
Adobe PDF
Checksum
(MD5):682fcc50616f04948c56983f3005491d