Chemical identification of a sulfated glucan from Antrodia cinnamomea and its anti-cancer functions via inhibition of EGFR and mTOR activity
Journal
Carbohydrate Polymers
Journal Volume
202
Pages
536-544
Date Issued
2018
Author(s)
Lu, M.-K.; Lin, T.-Y.
Abstract
Antrodia cinnamomea is a polyporaceous medicinal and native fungus in Taiwan. In this study, we found that AC-SPS-F3, a sulfated glucan from A. cinnamomea, reduced lung cancer cell viability via inhibition of EGFR and mTOR activity. The co-administration of AC-SPS-F3 and cisplatin synergistically inhibited lung cancer cell viability. We identified AC-SPS-F3 was a sulfated β-(1→4)-D-glucan with two long 1,6-branches in each repeat unit. The FT-IR absorption at 1341 cm–1 and 887 cm–1 confirmed the existence of sulfates. The proposed repeat unit of AC-SPS-F3, including the types of main skeleton and side chains, as well as the position of the minor galactopyranosyl and mannopyranosyl residues, were proposed according to the 1D and 2D NMR spectra, shown as follows: The features for the proposed repeat unit of AC-SPS-F3 included two long β-(1→6)-Glcp branches, a very high ratio of sulfate substitution, and partial 2-O and 4-O substituents evenly distributed on the β-(1→6)-Glcp branches. The present study is first to characterize the highly branched sulfated polysaccharides and elucidates its anti-cancer functions. [Figure presented] ? 2018 Elsevier Ltd
Subjects
Antrodia cinnamomea; FT-IR; Lung cancer; NMR; Sulfated polysaccharides; Synergistic effect
SDGs
Other Subjects
Antibiotics; Biological organs; Cells; Nuclear magnetic resonance; Nuclear magnetic resonance spectroscopy; Polysaccharides; Sulfur compounds; 2D NMR spectra; Antrodia cinnamomea; Chemical identification; IR absorption; Lung Cancer; Lung cancer cells; Sulfated polysaccharides; Synergistic effect; Diseases; antineoplastic agent; EGFR protein, human; epidermal growth factor receptor; glucan; MTOR protein, human; sulfate; target of rapamycin kinase; antagonists and inhibitors; Antrodia; cell proliferation; cell survival; chemistry; conformation; drug effect; drug screening; human; isolation and purification; metabolism; tumor cell culture; Antineoplastic Agents; Antrodia; Carbohydrate Conformation; Cell Proliferation; Cell Survival; Drug Screening Assays, Antitumor; ErbB Receptors; Glucans; Humans; Sulfates; TOR Serine-Threonine Kinases; Tumor Cells, Cultured
Type
journal article