Proteomic analysis and identification of aqueous humor proteins with a pathophysiological role in diabetic retinopathy
Journal
Journal of Proteomics
Journal Volume
75
Journal Issue
10
Pages
2950-2959
Date Issued
2012
Author(s)
Chiang S.-Y.
Tsai M.-L.
Chen A.
Chou Y.-C.
Hsia C.-W.
Wu Y.-F.
Chen H.-M.
Huang T.-H.
Chen P.-H.
Liu H.-T.
Shui H.-A.
Abstract
Diabetic retinopathy (DR) can cause irreversible blindness and is the severest microvascular complication in the eyes of patients with diabetic mellitus (DM). The identification of susceptibility factors contributing to development of DR is helpful for identifying predisposed patients and improving treatment efficacy. Although proteomics analysis is useful for identifying protein markers related to diseases, it has never been used to explore DR-associated susceptibility factors in the aqueous humor (AH). To better understand the pathophysiology of DR and to identify DR-associated risk factors, a gel-based proteomics analysis was performed to compare AH protein profiles of DM patients with and without development of DR. MALDI-TOF MS was then performed to identify protein spots that were differentially expressed between the two groups and western blot analysis was used to validate the expressional change of protein demonstrated by proteomics. Our proteomics and bioinformatics analysis identified 11 proteins differentially expressed between DR and control groups. These proteins are linked to biological networks associated with nutrition transport, microstructure reorganization, angiogenesis, anti-oxidation, and neuroprotection. The data may provide potential AH biomarkers and susceptibility factors for predicting DR development, and provide an insight into the underlying pathophysiological mechanisms of DR. This article is part of a Special Issue entitled: Proteomics: The clinical link. ? 2011 Elsevier B.V..
Other Subjects
apolipoprotein A1; brain specific angiogenesis inhibitor 1 associated protein 2; collagenase 3; cystathionine beta synthase; growth factor receptor bound protein 10; keratin type i cytoskeletal 10 protein; keratin type i cytoskeletal 9 protein; podocan; protein; retrotransposon gag domain containing protein 1; selenoprotein P; transferrin; unclassified drug; adult; aged; aqueous humor; article; clinical article; controlled study; diabetic retinopathy; human; matrix assisted laser desorption ionization time of flight mass spectrometry; neuroprotection; pathophysiology; priority journal; protein analysis; protein expression; proteomics; risk factor; Western blotting; Aged; Aged, 80 and over; Aqueous Humor; Case-Control Studies; Diabetic Retinopathy; Electrophoresis, Gel, Two-Dimensional; Eye Proteins; Humans; Metabolic Networks and Pathways; Middle Aged; Models, Biological; Osmolar Concentration; Proteome; Proteomics; Validation Studies as Topic
Type
journal article