行政院國家科學委員會專題研究計畫成果報告:製備豬霍亂沙門氏菌HTRA, AROA突變株及其做為活菌疫苗之黏膜免疫
Date Issued
1999
Date
1999
Author(s)
潘銘正
DOI
882313B002053
Abstract
Salmonella choleraesuis is an important
swine infectious disease. In order to produce
an attenuated Salmonella choleraesuis as a
vaccine, we expect to clone a htrA gene
mutated Salmonella choleraesuis to be a
attenuated strain. At first, we inactivate htrA
gene by inserting a karamycine resistant gene
to the htrA gene containing fragment which
is amplified by PCR with primers derived
from Salmonella typhimurium htrA gene and
then ligate the mutated htrA gene to suicide
plasmid pGP704. After transforming to
Salmonella choleraesuis, we have selected
several colonies. Unfortunately, we are
unable to amplify the inactivated htrA gene
fragment from all the colonies.
Subjects
Salmonella choleraesuis
htra
gene
gene
Salmonella typhimurium
PCR
SDGs
Publisher
臺北市：國立臺灣大學獸醫學系暨研究所
Type
report
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