Quantitative Phosphoproteomic Analysis Reveals the Regulatory Pathways of ZNF322A in Human Lung Adenocarcinoma A549 Cells
Date Issued
2015
Date
2015
Author(s)
Chen, Wei-Ting
Abstract
ZNF322A is a transcription factor and belongs to the krüppel C2H2-type zinc-finger protein family. Recently, ZNF322A was reported as a potential oncogene in lung cancer patients and is critical for tumorigenesis. However, ZNF322A-mediated downstream signaling pathways in lung cancer cells remain unclear. Using hydroxy acid-modified metal oxide chromatography (HAMMOC) and nanoscale liquid chromatography–tandem MS (nanoLC-MS/MS), we examined protein phosphorylation induced by ZNF322A signaling in lung cancer A549 cells. We identified 4501 phosphorylation sites in 1309 phosphoproteins. Among these phosphosites, 443 were significantly changed in response to ZNF322A silencing. Using bioinformatics approaches including functional enrichments, network analysis and phosphorylation motif analysis, we highlighted a previously unidentified ZNF322A-mTOR signaling pathway and autophagic process. On the other hand, we demonstrated that ZNF322A plays an important role in cancer progression such as cytoskeleton regulation and tumor formation-associated process. This study not only gives new information about the molecular regulation by ZNF322A at post-translational level, but also provides a resource for the study of lung cancer therapy.
Subjects
Zinc-finger protein
oncogene
phosphoproteome
HAMMOC
autophagy
SDGs
Type
thesis
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