3D-Printed proangiogenic patches of photo-crosslinked gelatin and polyurethane hydrogels laden with vascular cells for treating vascular ischemic diseases
Journal
Biomaterials
Journal Volume
309
ISSN
0142-9612
Date Issued
2024-09
Author(s)
Yu-Tsung Shih
Kun-Chih Cheng
Yi-Ju Ko
Chia-Yu Lin
Mei-Cun Wang
Chih-I Lee
Pei-Ling Lee
Rong Qi
Jeng-Jiann Chiu
DOI
10.1016/j.biomaterials.2024.122600
Abstract
Engineering vascularized tissues remains a promising approach for treating ischemic cardiovascular diseases. The availability of 3D-bioprinted vascular grafts that induce therapeutic angiogenesis can help avoid necrosis and excision of ischemic tissues. Here, using a combination of living cells and biodegradable hydrogels, we fabricated 3D-printed biocompatible proangiogenic patches from endothelial cell-laden photo-crosslinked gelatin (EC-PCG) bioink and smooth muscle cell-encapsulated polyurethane (SMC-PU) bioink. Implantation of 3D-bioprinted proangiogenic patches in a mouse model showed that EC-PCG served as an angiogenic capillary bed, whereas patterned SMC-PU increased the density of microvessels. Moreover, the assembled patterns between EC-PCG and SMC-PU induced the geometrically guided generation of microvessels with blood perfusion. In a rodent model of hindlimb ischemia, the vascular patches rescued blood flow to distal tissues, prevented toe/foot necrosis, promoted muscle remodeling, and increased the capillary density, thereby improving the heat-escape behavior of ischemic animals. Thus, our 3D-printed vascular cell-laden bioinks constitute efficient and scalable biomaterials that facilitate the engineering of vascular patches capable of directing therapeutic angiogenesis for treating ischemic vascular diseases.
Subjects
3D-bioprinted proangiogenic patch
Cell-laden bioink
Ischemic disease
Photo-crosslinked gelatin
Polyurethane
SDGs
Publisher
Elsevier BV
Type
journal article