Testosterone regulation on quiescin sulfhydryl oxidase 2 synthesis in the epididymis
Journal
Reproduction
Journal Volume
161
Journal Issue
5
Pages
593-602
Date Issued
2021
Author(s)
Abstract
The epididymis is an androgen-responsive organ, whose structure and functions are modulated by the coordination between androgen and epididymal cues. Highly regulated molecular interaction within the epididymis is required to support viable sperm development necessary for subsequent fertilization. In the present study, we extended our earlier findings on a promising epididymal protein, quiescin sulfhydryl oxidase 2 (QSOX2), and demonstrated a positive correlation between testosterone and QSOX2 protein synthesis through the use of loss- and restore-of-function animal models. Moreover, based on transcriptomic analyses and 2D culture system, we determined that an additional polarized effect of glutamate is indispensable for the regulatory action of testosterone on QSOX2 synthesis. In conclusion, we propose noncanonical testosterone signaling supports epididymal QSOX2 protein synthesis, providing a novel perspective on the regulation of sperm maturation within the epididymis. ? 2021 Society for Reproduction and Fertility
Subjects
glutamic acid
messenger RNA
quiescin sulfhydryl oxidase 2
testosterone
thiol oxidase
unclassified drug
carrier protein
glutamate transporter
glutamine
glutamine transport proteins
oxidoreductase
Qsox2 protein, mouse
animal cell
animal tissue
Article
controlled study
epididymis
hormonal regulation
male
mouse
nonhuman
priority journal
protein expression
protein synthesis
RNA sequencing
signal transduction
spermatozoon maturation
transcriptomics
two dimensional cell culture
animal
cytology
drug effect
gene expression regulation
genetics
Institute for Cancer Research mouse
metabolism
Amino Acid Transport System X-AG
Animals
Carrier Proteins
Epididymis
Gene Expression Regulation
Glutamic Acid
Glutamine
Male
Mice
Mice, Inbred ICR
Oxidoreductases Acting on Sulfur Group Donors
Sperm Maturation
Testosterone
SDGs
Type
journal article