Bioaugmented hydrogen production from microcrystalline cellulose using co-culture-Clostridium acetobutylicumX9 and Ethanoigenens harbinenseB49
Journal
International Journal of Hydrogen Energy
Journal Volume
33
Journal Issue
2
Pages
912-917
Date Issued
2008
Author(s)
Abstract
Dark fermentation of microcrystalline cellulose to produce biohydrogen using mono-culture or co-culture of isolated strains was studied. A strain (X9) with high hydrogen yield from microcrystalline cellulose was isolated and identified to be closely affiliated with Clostridium acetobutylicum, ATCC 824. At 37 {ring operator} C and pH 5.0, the mono-culture of X9 yields hydrogen with a 5-h time lag and end liquid products primarily of acetate and butyrate. The co-culture of X9 with another strain, Ethanoigenens harbinenseB49, which can produce hydrogen efficiently from monosaccharides but directly from microcrystalline cellulose, produced more efficiently the biohydrogen via ethanol-type fermentation metabolism compared with mono-culture X9 test. Bioaugmentation with X9 + B49 improved cellulose hydrolysis and subsequent hydrogen production rates as compared with that of mono-culture bioaugmentation with X9. ? 2007 International Association for Hydrogen Energy.
Subjects
Bioaugmentation
Biohydrogen
Co-culture
Microcrystalline cellulose
SDGs
Other Subjects
Cell culture; Cellulose; Hydrolysis; Bioaugmentation; Biohydrogen; Hydrogen production; Cells; Hydrogen; Hydrolysis; MicrOCRystalline Cellulose
Type
journal article