Effect of A-scan rate and interscan interval on optical coherence angiography
Journal
Biomedical Optics Express
Journal Volume
12
Journal Issue
2
Pages
722-736
Date Issued
2021
Author(s)
Abstract
Optical coherence tomography angiography (OCTA) can provide rapid, volumetric, and noninvasive imaging of tissue microvasculature without the requirement of exogenous contrast agents. To investigate how A-scan rate and interscan time affected the contrast and dynamic range of OCTA, we developed a 1.06-?m swept-source OCT system enabling 100-kHz or 200-kHz OCT using two light sources. After system settings were carefully adjusted, almost the same detection sensitivity was achieved between the 100-kHz and 200-kHz modalities. OCTA of ear skin was performed on five mice. We used the variable interscan time analysis algorithm (VISTA) and the designated scanning protocol with OCTA images reconstructed through the correlation mapping method. With a relatively long interscan time (e.g., 12.5 ms vs. 6.25 ms for 200-kHz OCT), OCTA can identify more intricate microvascular networks. OCTA image sets with the same interscan time (e.g., 12.5 ms) were compared. OCTA images acquired with a 100-kHz A-scan rate showed finer microvasculature than did other imaging modalities. We performed quantitative analysis on the contrast from OCTA images reconstructed with different A-scan rates and interscan time intervals in terms of vessel area, total vessel length, and junction density. ? 2021 Optical Society of America under the terms of the OSA Open Access Publishing Agreement
Subjects
Angiography; Image analysis; Light sources; Mammals; Optical tomography; Contrast agent; Correlation mapping method; Detection sensitivity; Imaging modality; Micro-vasculature; Microvascular network; Non-invasive imaging; Swept source OCT; Image reconstruction; A scan; algorithm; animal experiment; article; controlled study; ear; human; intermethod comparison; light; male; microvasculature; mouse; nonhuman; optical coherence tomography angiography; quantitative analysis; skin
SDGs
Publisher
OSA - The Optical Society
Type
journal article