以螢光二次元差異性電泳及質譜儀來分析肝癌與其相對應的非癌肝組織，蛋白質表現的差異
Date Issued
2004
Date
2004
Author(s)
許金川
DOI
922314B002143
Abstract
Hepatocellular carcinoma (HCC) has been the leading cause of cancer death in
Taiwan. About 6000-8000 people died of this cancer every year in Taiwan. Though
regular sonographic examination can early detect small HCC and there are many
therapeutic modalities for HCC, the therapeutic results remains unsatisfactory. To
improve the survival, further investigation of the early diagnostic markers and the
mechanisms of hepatocarcinogenesis is very important. In the recent years, investigating the genome-wide expression profiles of cancers has
been the predominant method to identify cancer-related genes. Though using cDNA
microarray for genome-wide expression profiling is a very powerful tool to clarify the
genetic changes in cancers, the major pitfall of these methods is that the mRNA
expression does not parallel protein expression in many cases.
The introduction of fluorescent 2D differential gel electrophoresis (DIGE) has
now made it possible to detect and quantitate differences between experimental pairs of
samples resolved on the same 2D gel. The basis of this technique is to use two
fluorescent dyes (Cy3 and Cy5) to differentially label lysine residues of two protein
samples for comparative analysis on a single gel. The ability to directly compare two
samples on the same gel not only avoids the complications of gel-to-gel variation but also
enables a more accurate and rapid analysis of differences and reduces the number of gels
that need to be run. Following automated image analysis, using the novel and innovative
software, spots of interest are selected for gel excision, subjected to in-gel enzymatic
digestion, and mass spectrometry identification.
In this current project, we enrolled eight paired of HCC and the corresponding
non-tumor liver tissues, and subjected to DIGE analysis. We found that 9 proteins (eg.
heat shock protein) were upregulated in the HCC tissues, while 11 proteins were
downregulated in the HCC tissues. We are currently to investigate whether these proteins
could be used as the new HCC diagnostic markers.
Subjects
hepatocellular carcinoma
proteomics
differential gel electrophoresis
DIGE
MALDI
mass spectrometry
SDGs
Publisher
臺北市：國立臺灣大學醫學院內科
Type
report
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