Determination of Aflatoxin M1 in Milk and Milk Powder Using Solid-phase Extraction and High-performance Liquid Chromatography／Tandem Mass Spectrometry

Milk may contain aflatoxin M1 (AFM1) if cows ingest aflatoxin B1（AFB1）, which is a usual contaminant in animal feed. AFM1 is toxic and carcinogenic to liver, and can survive after pasteurization. The European Union has reduced the regulated level down to 0.05 μg/L and has challenged the existing analytical methods.
To confirm the existence of aflatoxin M1, the most used measurement is by high-performance liquid chromatography (HPLC)/fluorescence detection. Usually derivatization is needed to enhance the fluorescence of aflatoxin M1, which needs toxic reagent and additional time. In terms of sample preparation, the sample size is limited to 20