DNA detection using functionalized conducting polymers
Journal
Methods in Molecular Biology
Journal Volume
751
Pages
437-452
Date Issued
2011
Author(s)
Abstract
A well-defined DNA bioconjugated surface is a key component in the development of efficient biosensor platforms for diseases, ranging from point-of-care detection of pathogens and viruses to personalized diagnostics and medication, as well as for drug discovery, forensics, and food technology. We herein describe a universal and rapid methodology to construct such surfaces based on functionalized conducting polymer thin films. The conducting polymers combine sensing properties with the ability to act as signal transducers for the biorecognition event. We have shown that biosensor designs based on conducting polymers display a number of advantageous features, such as a long-term stability, label-free sensing, fast analysis, and the capability to apply both electrochemical and fluorescent protocols for DNA detection.
SDGs
Type
book part