The study on wilt diseases of chrysanthemum and their control
Date Issued
2015
Date
2015
Author(s)
Yen, Chia-Ling
Abstract
Chrysanthemum Dendranthema grandiflorum (Ramat.) Kitam is a perennial herb, whose head inflorescence can be used as herbal medicine or herb drinks after drying process, and it has been reported to have a wide range of health benefits. Chrysanthemum in Taiwan is grown mainly in Miaoli and Taitung. Chrysanthemum can be affected by many diseases; over the last few years, the wilt disease of chrysanthemum caused serious yield loss in Miaoli and Taitung regoins. Over 10 percent of chrysanthemum withered in the field, especially in continuous cropping gardens, where the disease spreads seriously. To investigate the the causing agent of chrysanthemum wilt in Taiwan, several isolates of Fusarium spp. and bacteria were obtained from diseased plants collected from Miaoli chrysanthemum gardens in this study. The results of pathogenicity test showed that the bacteria isolates had pathogenicity while the Fusarium spp. did not. Though the colony phenotype of bacteria isolates on the TTC medium was similar to Ralstonia solanacearum, the molecular sequencing identification confirmed that the cause of chrysanthemum wilt should be Burkholderia gladioli. However, the possibility of co-infection with Fusarium cannot be excluded. Three antibiotics were chosen to control the chrysanthemum wilt in this study, and the results showed that there were no significant difference between the water control and the antibiotic treatments in a pot assay. The applications of three microbial agents for controlling the chrysanthemum wilt disease showed Bacillus subtilis WG6-14 had better control effect than the other treatments. Next, artemisia and Yue-Shan chrysanthemum were chosen to screen for resistance rootstocks. Both artemisia and Yue-Shan chrysanthemum grafted plants showed no wilt symptom after inoculation by a root-dipping method. The grafted plants of the sterile water control treatments and inoculated treatments by the root-dipping method presented no wilt symptom. According to these results, the grafting method has great potential as a control mean for chrysanthemum wilt.
Subjects
chrysanthemum wilt disease
bacterial wilt
microbial agent control
antibiotic control
grafting control
Type
thesis
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