Integration of high-risk human papillomavirus DNA correlates with HLA genotype aberration and reduced HLA class I molecule expression in human cervical carcinoma
Journal
Clinical Immunology
Journal Volume
115
Journal Issue
3
Pages
295-301
Date Issued
2005
Author(s)
Chiou S.-H.
Huang S.-C.
Hsu S.-M.
Abstract
In human cervical cancer (CC), local immunity against this human papilloma virus (HPV)-associated neoplasia has been signified. To stratify the possibility of HPV integration on HLA mutations, we measured the genotypic and phenotypic integrity of all available HLA class I loci in 30 cases of CC. Paired normal and cancer genomic DNA was analyzed with DNA typing trays, including 57 subtypes of HLA-A, 120 subtypes of HLA-B, and 60 subtypes of HLA-C. We demonstrated significant mutations of HLA genotype with reduced HLA molecule expression in CC. HPV coincide in >70% cases of aberrant HLA genes. Southern blot analysis revealed the presence of HPV DNA within the mutated HLA foci. Our study reveals a plausible role of HPV integration in the contexts of aberrant HLA genotypes in CC cells. Disruptions of the HLA genes can be possible tactics of HPV to attain the potential carcinogenetic purposes, and thus the cancer immune escape. ? 2005 Elsevier Inc. All rights reserved.
SDGs
Other Subjects
HLA A antigen; HLA antigen class 1; HLA B antigen; HLA C antigen; virus DNA; antigen expression; article; cancer genetics; cancer risk; carcinogenesis; controlled study; disease association; DNA determination; female; gene disruption; gene locus; gene mutation; genetic susceptibility; genotype; HLA system; human; human tissue; phenotype; priority journal; Southern blotting; tumor immunity; tumor promotion; uterine cervix carcinoma; virus virulence; Wart virus; Carcinoma; DNA, Viral; Down-Regulation; Female; Genes, MHC Class I; HLA Antigens; Humans; Mutation; Papillomaviridae; Phenotype; Uterine Cervical Neoplasms; Virus Integration
Type
journal article