Actin3 promoter reveals undulating F-actin bundles at shanks and dynamic F-actin meshworks at tips of tip-growing pollen tubes
Journal
Plant Signaling and Behavior
Journal Volume
11
Journal Issue
3
ISSN
15592316
Date Issued
2016-03-03
Author(s)
Abstract
ABSTRACT: The dynamic actin cytoskeleton of pollen tubes is both the driver of the tip growth and the organizer of cell polarity. In order to understand this fast re-arranging cytoskeletal system, we need reliable constructs expressed under relevant promoters. Here we are reporting that the Lifeact reporter, expressed under the pollen-specific Actin3 promoter, visualizes very dynamic F-actin elements both in germinating pollen grains and tip-growing pollen tubes. Importantly, we have documented very active actin polymerization at the cell periphery, especially in the bulging area during pollen germination and in the apical clear zone. Expression of the Lifeact reporter under control of the pollen-specific Actin3 promoter revealed 2 new aspects: (i) long F-actin bundles in pollen tube shanks are dynamic, showing undulating movements, (ii) subapical ‘actin collars’ or ‘fringes’ are absent. © 2016 Taylor & Francis Group, LLC.
Subjects
Actin cytoskeleton
actin3 promoter
lifeact construct
polarity
pollen tubes
Publisher
Taylor and Francis Inc.
Description
Article Number: e1146845
Type
journal article